anti dl mouse  (Developmental Studies Hybridoma Bank)

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Article Title: Whole-Tumor Clearing and Imaging of Intratumor Microbiota in Three Dimensions with miCDaL Strategy.

doi: 10.1002/advs.202400694

Figure Lengend Snippet: Figure 4. 3D views of spatial distribution between intratumor microbiota and activated T-cells. a) Schematic diagram showing the workflow of 3D imaging of native intratumor microbiota and related tumor immune microenvironment by the integrated antibody immunostaining. b) 3D view of a whole tumor from MMTV-PyMT mice. Activated T-cells are indicated by CD4 and CD8 antibodies. Intratumorally injected TADA was used for the in vivo labeling of intratumor microbes. Blue: CD4, Yellow: TADA, Red: CD8, White: DAPI. Scale bar, 2000 μm. Representative results from three independent experiments are shown.

Article Snippet: Antibody Final Concentration Ex/Em (nm) Brand Cat No. DyLight 488 Labeled Lycopersicon Esculentum (Tomato) Lectin (LEL, TL) 1:1000 488/510 VECTOR DL-1174-1 Brilliant Violet 510 anti-mouse CD4 1:500 405/510 Biolegend 100449 Alexa Fluor 647 anti-mouse CD8a 1:500 633/660 Biolegend 100724 PE anti-human CD56 (NCAM) 1:200 550/570 Biolegend 304605 PE anti-human CD3 1:200 550/570 Biolegend 300307 FITC anti-human CD23 1:200 488/520 Biolegend 338506 immune infiltration at Z-depths of 2.5–5.5 mm.

Techniques: Imaging, Immunostaining, Injection, In Vivo, Labeling